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Module 1:

Basic A-scan Biometry   
 

Section 1:

Basic Concepts 
     
 

Introduction

Reflectivity

Perpendicularity

Gain level

Optical axis

Spike height

Corneal compression

Velocity of sound

 
   
 

Introduction

A-scan biometry is also called an axial length measurement, or simply an "A-scan" or "A's".  This measurement is combined with keratometry ("A's and K's") in a formula to determine the power of the intraocular lens that replaces the natural lens that is removed from the eye during cataract surgery.

Accuracy is important.  A .4mm error in the measurement may result in a one diopter error in calculated IOL power. This module covers concepts and techniques necessary to arrive at an accurate measurement.

Reflectivity

Reflection from dissimilar substances The A-scan probe projects a thin sound beam that travels through liquid or tissue.  When the sound beam encounters the interface of a substance that is dissimilar from the substance it is traveling through, part of the sound beam energy is reflected, and part of the sound energy projects through the new substance.   The more dissimilar the substances are, the stronger the reflection, or echo, is.

 

A-scan graphWhen the A-scan beam is projected into the phakic (natural lens) eye it travels through the aqueous humor and encounters a dissimilar substance in the anterior lens surface.  Energy is reflected back to the transducer in the probe tip.  The instrument displays the intensity of the reflection as a spike above the baseline (which represents distance) on a graph.

Strong reflections also occur as the sound beam encounters the posterior lens surface and the retina.  Spikes representing these reflections appear at their corresponding positions along the baseline.  The first spike represents the probe tip as it comes into contact with the cornea.

Perpendicularity

Improper alignmentEven a highly reflective structure such as the retina will produce a relatively low spike if the sound beam strikes the retinal surface at an angle.

If the sound beam is aimed off axis, the beam will hit a highly reflective surface at an angle and sound energy is reflected away from the transducer, producing low spikes on the baseline.

The highest spikes occur when the beam strikes a highly reflective object in a perpendicular orientation.

Gain level

The affect of gain on the spike height.Gain is the electronic amplification of the signal coming back to the probe.  There is usually a manual control that can be changed by the operator.   Some instruments adjust the gain automatically.

For a given eye and a given probe orientation, the higher the gain level is, the higher the spikes will be above the baseline.

 

Optical axis

The optical axisThe optical axis of the eye is the distance from the corneal apex to the fovea.  This is also the longest cornea-to-retina distance in the normal eye.

 

 

The optical axis and perpendicularityA light beam, or ultrasound beam, passing along the optical axis of the eye will encounter the anterier lens capsule, the posterior lens capsule, and the retina in a perpendicular orientation.

 

 

Spike height

Beam aligned with axis, resulting in tall spikesAs discussed earlier in this section, the height of A-scan graph spikes are affected by the reflectivity of interfaces the beam encounters, the orientation of the beam to substances in its path (perpendicularity), and by the gain level of the ultrasound instrument.

Because of these factors, spike height becomes an important indicator as to the accuracy of the A-scan.  Tall lens and retina spikes indicate that the ultrasound beam is striking these structures in a perpendicular fashion, giving an indication that the beam is aligned with the optical axis of the eye.

Corneal compression

Corneal compression.Since ultrasound does not travel through air,  the A-scan probe must come into contact with the cornea, either directly or through a liquid.   If a corneal-contact method is used, there is a danger of the probe putting too much pressure on the cornea, causing the cornea to compress, which results in an artificial shortening of the axial eye length.

This is a significant potential error source.  A .4mm compression error can result in a 1 diopter error in the calculated IOL power.

Velocity of Sound

Ultrasound travels at different speeds through materials of different densities.  Ultrasound is conducted very well through water and materials containing  a large percentage of water.  Ultrasound waves do not travel through air.

The denser the material is, the faster sound travels through it.   A-scan instruments compute distances by measuring the time it takes ultrasound to travel through the structures of the eye.  More specifically, the time it takes an ultrasound beam to travel from the probe, bounce off of an object, and return to the probe.

The following are the speeds of ultrasound through the structures of the eye:

  • cornea -  1640 m/s  (meters per second)
  • aqueous - 1532 m/s
  • normal lens - 1640 m/s
  • cataractous lens - 1629 m/s
  • vitreous - 1532 m/s

Many A-scan instruments do not measure each individual segment in the eye.  Instead they use an average velocity of sound.  Many use 1550 for a phakic eye and 1532 for an aphakic eye.

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